Cellular Immunostimulation by CpG-Sequence-Coated DNA Origami Structures
نویسندگان
چکیده
منابع مشابه
RNA-templated DNA origami structures.
Using the RNA transcript as a template, RNA-templated DNA origami structures were constructed by annealing with designed DNA staple strands. RNA-templated DNA origami structures were folded to form seven-helix bundled rectangular structures and six-helix bundled tubular structures. The chemically modified RNA-DNA hybrid origami structures were prepared by using RNA templates containing modified...
متن کاملCpG Motif in Phage Genome DNA Enhanced the Efficacy of Phage Therapy by Immunostimulation
Lytic phages for multiple-drug resistant E. coli and Pseudomonas aeruginosa were isolated. They belong to Siphoviridea and Myoviridea families, respectively. Lytic life cycle of the phage has therapeutic implications. Concurrent immune-stimulation of adaptive immunity against the etiologic agent, the MDR bacteria in the victim would revolutionize the field of phage therapy. In this pretext, met...
متن کاملCellular delivery of enzyme-loaded DNA origami.
In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a comp...
متن کاملMicrofabricating 3D structures by laser origami
Digital microfabrication techniques such as laser direct-write (LDW) offer a viable alternative for generating 3D self-folding designs. These designs enable novel electronic and optical functionality within limited form factors not possible via current 2D structures. The approach requires self-folding at the microscale where manual or mechanized actuation of the smaller structures is not practi...
متن کاملDielectrophoresis of gold nanoparticles conjugated to DNA origami structures
DNA nanostructures are promising construction materials to bridge the gap between self-assembly of functional molecules and conventional top-down fabrication methods in nanotechnology. Their positioning onto specific locations of a microstructured substrate is an important task towards this aim. Here we study manipulation and positioning of pristine and of gold nanoparticle-conjugated tubular D...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Nano
سال: 2011
ISSN: 1936-0851,1936-086X
DOI: 10.1021/nn203161y